Phylogenetic Study of Mangifera Central Sumatra Based on rbcl Sequences

Fitmawati ., Erwina Juliantari, Nery Sofiyanti

Abstract


Mangifera from Central Sumatra has a unique ability to adapt to the high rainfall region. Therefore this genus is a potential germplasm source in Sumatera. However the diversity of Mangifera was decreasing due to many factors such as deforestation and habitat changes. Those factors may cause loss information of Mangifera whereas its diversity and taxonomical status were not yet clear. The aim of this study was to reconstruct the relationship of Mangifera based on the rbcL sequences. DNA was isolated from leaves using CTAB method. DNA sequencing was done in First Laboratories, Malaysia. The phylogenetic reconstruction was conducted using PAUP* Version 4.0b10 with Maximum Parsimony (MP) and Neighbor Joining (NJ) analysis. Cladogram using MP showed monophyletic tree and was divided into two main clades. The first clad only consist of M. casturi and the second clad consist of M. indica, M.  laurina, M.  sumatrana, M.  quadrifida, M. odorata, M. kemanga, Mamgifera sp. and M.  torquenda. The NJ cladogram supported MP analysis and showed that Mangifera sp. has  the highest genetic distance to other species.

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