Flash SiO2 Liquid Chromatography Fractionation of Garcinia mangostana cellulase-Ethanol Extracts & Antioxidant Activity of Each Fractions

Sarah Fadhilah Zaili, Yum Eryanti, Hilwan Yuda Teruna, Titania T. Nugroho

Abstract


The mangosteen fruit rind (Garcinia mangostana Linn) contains phenolic compounds and derivatives that have antioxidant activity. Mangosteen rind was extracted with T. asperellum LBKURCC1 cellulase in 0.05 M sodium acetate buffer pH 5.5 and 50% ethanol. This extract showed high antioxidant activity but was toxic to skin cell cultures caused by the high salt contained in the concentrated extract. The purpose of this study was to eliminate the salts contained in the extract by SiO2flash liquid chromatography (LC) and determine the antioxidant activity in fractions obtained. Ten fractions were collected and concentrated by rotary evaporator at room temperature and further waterbath dried at 50°C to constant weight. Thin layer chromatography (TLC) analysis of the concentrated extracts indicated that there were several components not yet separated in each fraction. Antioxidant activity of each fraction was determined by the DPPH (2,2-diphenyl-1-picrylhidrazyl) method. Test showed that the antioxidant activity obtained from all 10 fractions were significantly lower (p<0.05) than the original extract with IC50 values >1000 µg/mL. The original extract had an IC50  of 15.73 µg/mL.


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