Antimicrobial Activity of Secondary Metabolites from Liquid Fermentation Medium of Penicillium sp. LBKURCC34 Stimulated by Staphilococcus aureus

Khairullinas Khairullinas, Citra Hardiyanti, Ayu Putri Anugrah, Yuana Nurulita

Abstract


Natural products derived from microbes are potentially useful in numerous fields especially in industry and medicine. Considered from the medical point of view, microbial metabolites have been the prime source of most antibiotics currently available on the market. However, there is an alarming decrease in newly discovered antibiotics during the past decades. This is not a good sign considered that there is an occurrence of multi-drug resistant organisms and newly identified pathogens. Fungi represent an interesting group known to produce a wide range of bioactive compounds. Fungal metabolites are diverse in terms of structures and functions. Penicillium sp. LBKURCC34 is a fungi isolated from peat soil of primary forest at Giam Siak Kecil Bukit Batu (GSKBB), Biosphere Reserve in Riau Province. The purpose of this study is to produce secondary metabolites that have an antimicrobial activity from the growth media of Penicillium sp. LBKURCC34. Secondary metabolite production was done by fermentation in a liquid medium inoculated with spores of Penicillium sp. LBKURCC34 (7x1012 spores in 50 mL of media). The fermentation was carried out for 14 days in the media and extracted with ethyl acetate. The ethyl acetate crude extract was evaporated, then the concentrate dissolved methanol. Phytochemicals test was applied to the extract. Antifungal test was performed by the disc diffusion method toward Eschericia Coli, Bacillus Subtilis, Staphilococcus Aereus, Staphilacoccus Ephidermidis, and Candida albicans growth as microbial pathogen using 3 concentration of crude extracts (1.9; 3.8; and 5.7 mg/mL). The results of phytochemical test showed positive results for terpenoid. The crude extract inhibited all these pathogen. Crude extract identification was performed on Thin Layer Chromatography (TLC) with eluent of hexane: ethyl acetate (1:1) giving two spots under UV (254 nm), and on High Performance Liquid Chromatography (HPLC) giving three peaks under UV detector (254 nm).




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